Fig. 5: SORT1 is a target gene of miR-127-5p and miR-136-5p. | Cell Death & Disease

Fig. 5: SORT1 is a target gene of miR-127-5p and miR-136-5p.

From: Circular RNA hsa_circ_0110389 promotes gastric cancer progression through upregulating SORT1 via sponging miR-127-5p and miR-136-5p

Fig. 5

A Computational prediction with Gene Expression Omnibus (GEO, GSE106656) and TargetScan to identify the overlapped potential target genes of miR-127-5p and miR-136-5p. B, C The mRNA and protein expression levels of six overlapped target genes in HGC-27 and KATO III cells with hsa_circ_0110389 knockdown by qRT-PCR and western blot. D The mRNA level of SORT1 in HGC-27 and KATO III cells with miR-127-5p or miR-136-5p mimics by qRT-PCR. E The protein levels of SORT1 and autophagy-related genes in HGC-27 and KATO III cells with miR-127-5p or miR-136-5p mimics by western blot. F The mRNA level of SORT1 in AGS and NCI-N87 cells with miR-127-5p or miR-136-5p inhibitors by qRT-PCR. G The protein levels of SORT1 and autophagy-related genes in AGS and NCI-N87 cells with miR-127-5p or miR-136-5p inhibitors by western blot. H RNA pull-down results showed that miR-127-5p and miR-136-5p may directly bind with SORT1. I, J (Up) The binding sites of wild type or mutant SORT1 3′-UTR with miR-127-5p (I) or miR-136-5p (J). (Down) Dual luciferase reporter assays demonstrated that SORT1 is a direct target of miR-127-5p (I) or miR-136-5p (J). K The expression of LC3B in HGC-27 and KATO III cells with miR-127-5p or miR-136-5p mimics by IF analysis. L The expression of LC3B in AGS and NCI-N87 cells with miR-127-5p or miR-136-5p inhibitors by IF analysis. All data are presented as mean ± SD. *p < 0.05, ** p < 0.01, ***p < 0.001.

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