Fig. 6: Hsa_circ_0110389 promotes the proliferation of GC cells through SORT1. | Cell Death & Disease

Fig. 6: Hsa_circ_0110389 promotes the proliferation of GC cells through SORT1.

From: Circular RNA hsa_circ_0110389 promotes gastric cancer progression through upregulating SORT1 via sponging miR-127-5p and miR-136-5p

Fig. 6

A, C, E, G, I HGC-27 and KATO III cells were transfected with sh-NC, sh-circ, sh-circ+EV or sh-circ+SORT1. Then the relative mRNA and protein expression of SORT1 was respectively measured by qRT-PCR analysis (A) and western blot assays (C), and the ability of cell proliferation and growth was assessed by CCK-8 assay (E), EdU assay (G) or colony formation assay (I). B, D, F, H, J AGS and NCI-N87 cells were transfected with pCD5-NC, pCD5-circ, pCD5-circ+shNC or pCD5-circ+shSORT1. Then the relative mRNA and protein expression of SORT1 was respectively measured by qRT-PCR analysis (B) and western blot assays (D), and the ability of cell proliferation and growth was assessed by CCK-8 assay (F), EdU assay (H) or colony formation assay (J). C, D The protein levels of autophagy-related genes (Beclin-1, LC3B and p62) were also analyzed by western blot assays. All data are presented as mean ± SD. EV empty vector. *vs sh-NC/pCD5-NC group, #vs EV/shNC group. **p < 0.01, ***p < 0.001, ##p < 0.01, ###p < 0.001.

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