Fig. 1: Higher USP35 expression is associated with ER+ breast cancer and predicts poor outcome. | Cell Death & Disease

Fig. 1: Higher USP35 expression is associated with ER+ breast cancer and predicts poor outcome.

From: USP35, regulated by estrogen and AKT, promotes breast tumorigenesis by stabilizing and enhancing transcriptional activity of estrogen receptor α

Fig. 1

a USP35 mRNA levels in tumor tissues are significantly higher than in normal tissues. p < 0.0001, two-tailed paired Student’s t-test. b USP35 mRNA level is higher in luminal than in other subtypes of breast cancer. **, p < 0.01; ***, p < 0.001; ns not significant, one-way ANOVA. c Gene Enrichment Set Analysis (GESA) showed that breast cancer with high USP35 mRNA level was enriched with the ESR1 up, luminal B up sets. d USP35 immunohistochemistry analysis showed that USP35 protein levels were higher in primary breast tumor tissues than in paired adjacent normal tissues. e Immunohistochemistry analysis showed that USP35 protein levels were significantly higher in ER+ than in ER− breast tumors (left). *, p < 0.05, two-tailed Student’s t-test. USP35 staining in different ER+ and ER− breast tumors were shown (right). Scale bar, 100 μm. Kaplan–Meier analysis for overall survival of breast cancer patients depending on USP35 mRNA levels in the TCGA database (f), USP35 amplification status in breast cancer (g), and in ER+ breast cancer (h) in the METABRIC database were shown. p value was calculated using the log-rank test.

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