Fig. 3: DIDO transcriptional termination and regulation of POU5f1 RNA. | Cell Death & Disease

Fig. 3: DIDO transcriptional termination and regulation of POU5f1 RNA.

From: Impaired stem cell differentiation and somatic cell reprogramming in DIDO3 mutants with altered RNA processing and increased R-loop levels

Fig. 3

a POU5f1 example gene: IGV image of the POU5f1 locus with parameters as in Fig. 2c, showing 3′UTR read-through in the DIDO3ΔE16 mutant. b Different abundance of POU5f1 short and long 3′UTR (indicated in a) confirmed in WT, DIDO3ΔE16, and DIDO3ΔCT+HADIDO3 RNA as tested by RT-PCR. c Scheme of expression plasmids: introducing 5′ HA-tag sequences and short or long 3′UTR to the open reading frame (ORF) of POU5f1 DNA. d Western blot analysis of lysates from ESC and embryonic bodies at d3 and d4 after LIF withdrawal plus retinoic acid from WT ESC transfected with (plasmids as in c), developed with anti-HA antibody; β-ACTIN was used as loading control.

Back to article page