Fig. 3: circPDE3B promotes ESCC cell proliferation and invasion in vitro.

A CCK-8 assay was used to detect cell viability of TE-1 and EC9706 cells after circPDE3B knocking down. B Colony formation assays were used to detect the clone ability of TE-1 and EC9706 cells after circPDE3B knocking down. C EdU assays were used to detect the DNA synthesis rate of TE-1 and EC9706 cells after circPDE3B knocking down. Scale bar: 50 μm. D Transwell assays were used to detect cell migration and invasion capacities of TE-1 and EC9706 cells after circPDE3B knocking down. E CCK-8 assays were used to detect cell viability of TE-1 and EC9706 cells after enforced expression of circPDE3B. F Colony formation assays were used to detect the clone ability of TE-1 and EC9706 cells after enforced expression of circPDE3B. G EdU assays were used to detect the DNA synthesis rate of TE-1 and EC9706 cells after enforced expression of circPDE3B. Scale bar: 50 μm. H Transwell assays were used to detect cell migration and invasion capacities of TE-1 and EC9706 cells after enforced expression of circPDE3B. *p < 0.05, **p < 0.01, ***p < 0.001.