Fig. 6: PCGF1 knockdown inhibits tumour growth in vivo.
From: PCGF1 promotes epigenetic activation of stemness markers and colorectal cancer stem cell enrichment
A, B Tumour size in nude mice after control and shPCGF1 HCT116 CRC cell injection on the day of harvest. C, D Tumour volume was measured every 3 days. Comparisons were performed for the shPCGF1 group versus the control group. E–H The histogram shows the average tumour weight and mouse weight of the two groups. n = 5 per group; error bars represent the SEM; **P < 0.01, *P < 0.05 versus control; one-way ANOVA and t-test. I Tumour sections were subjected to H&E staining and IHC using antibodies against PCGF1. H&E staining and IHC, scale bar, 20 µm. J, K Immunofluorescence staining of Ki-67 (red) and TUNEL (green) was performed to evaluate the proliferation of tumour cells. DAPI was used to stain the nuclei (blue). Ki-67 staining, scale bar, 20 µm; TUNEL staining, scale bar, 50 µm.
