Table 1 Primer sequences.
From: PCGF1 promotes epigenetic activation of stemness markers and colorectal cancer stem cell enrichment
Gene | Forward primer | Reverse primer |
|---|---|---|
β-Actin | TGGCACCACACCTTCTACAA | CCAGAGGCGTACAGGGATAG |
PCGF1 | GCCGGCTACTTCGTGGAT | GGTCCAGTTTGAGGTTGAGC |
CD133 | TGAACTGAGGCAGCTTCCACCC | CGACAGTCGTGGTTTGGCGTT |
CD44 | TTCATAGAAGGGCACGTGGT | CATTGGGCAGGTCTGTGAC |
ALDH1A1 | TGGAGTCAATGAATGGTGGAA | TGATTTGGCCACATACACCA |
Oct4 | GTCGCTCTCATCTTGCTCAATTCC | GGGATTGAGACATGCAGGAAGTG |
EZH2 | AATCAGAGTACATGCGACTGAGA | GCTGTATCCTTCGCTGTTTCC |
KDM6A | TTCCTCGGAAGGTGCTATTCA | GAGGCTGGTTGCAGGATTCA |
KDM6B | CGCTGCCTCACCCATATCC | ATCCGCGACCTCTGAACTCT |
KMT2A | ACTCAGTCTGGGGAATCTGC | TTGTCCTTCTCCACGCTCTT |
KMT2B | CCTTGGGACTCGAATCAGGT | GCAGCTTTGCCTCTTCCTTT |
KMT2C | CCCTTTCCAGAGAGCCAGAA | CCAACAGAGACCAGGGAGTT |
CD133 #1 (ChIP) | CCAGAAGCCGGGTCATAAATAAT | AGCGAACCCGTCCACTCCTCACT |
CD133 #2 (ChIP) | GAACTGCGGGGAGAGCGTGGTG | TCCCCGAGAGCGAGTCCGAAGTC |
CD44 (ChIP) | ATCTAGGTGTTCTAGCTCCTGAATC | CTACCATTCCTAGAGAAGGGAGTC |
ALDH1A1 (ChIP) | CTTGGTCCCTAGGTTCTCACAA | CACTTAAGCAGATCTTTTCTGCC |
