Table 2 Oligonucleotides used for plasmid construction.
From: PCGF1 promotes epigenetic activation of stemness markers and colorectal cancer stem cell enrichment
Plasmid constructs | |
PCGF1 | F: CCGCTCGAGATGGCGTCTCCTCAGG |
R: CGCGGATCCACCCTCCTCTTCTCTTTCACAC | |
Knockdown shRNA | |
PCGF1 sh#1 | F: CCGGGCAAGACAGTGAAGAGAAACGCTCGAGCGTTTCTCTTCACTGTCTTGCTTTTT |
R: AATTAAAAAGCAAGACAGTGAAGAGAAACGCTCGAGCGTTTCTCTTCACTGTCTTGC | |
PCGF1 sh#2 | F: CCGGGCAGCTTTGACCACTCTAAAGCTCGAGCTTTAGAGTGGTCAAAGCTGCTTTTT |
R: AATTAAAAAGCAGCTTTGACCACTCTAAAGCTCGAGCTTTAGAGTGGTCAAAGCTGC | |
PCGF1 sh#3 | F: CCGGGCCACTGCTCAACCTCAAACTCTCGAGAGTTTGAGGTTGAGCAGTGGCTTTTT |
R: AATTAAAAAGCCACTGCTCAACCTCAAACTCTCGAGAGTTTGAGGTTGAGCAGTGGC | |
PCGF1 sh#4 | F: CCGGGCTCCAGTCAGTGTACAAGATCTCGAGATCTTGTACACTGACTGGAGCTTTTT |
R: AATTAAAAAGCTCCAGTCAGTGTACAAGATCTCGAGATCTTGTACACTGACTGGAGC | |
