Fig. 2: CircSLIT2 boosted the aerobic glycolysis of PDAC cells. | Cell Death & Disease

Fig. 2: CircSLIT2 boosted the aerobic glycolysis of PDAC cells.

From: Novel circular RNA circSLIT2 facilitates the aerobic glycolysis of pancreatic ductal adenocarcinoma via miR-510-5p/c-Myc/LDHA axis

Fig. 2

A RT-qPCR showed the expression of circular form transcripts circSLIT2 and linear form transcripts in PDAC cell treated with RNase R. B RT-qPCR indicated the circular form transcripts circSLIT2 and linear form transcripts SLIT2 mRNA in PDAC cells administrated with actinomycin D. C The overexpression and silencing of circSLIT2 transfection were constructed using the plasmids or oligonucleotides transfection in Capan-2 and PANC-1 cells. D The abundance of glucose uptake was measured. E The production quantity measured by lactate analysis in Capan-2 and PANC-1 cells. F ATP analysis indicated the ATP output in PDAC cells. G Extracellular acidification rate (ECAR) assay and (H) oxygen consumption rate (OCR) assay demonstrated the glycolytic capacity of PDAC cells. **p < 0.01; *p < 0.05.

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