Fig. 4: MiR-510-5p served as the target of circSLIT2 in PDAC cells. | Cell Death & Disease

Fig. 4: MiR-510-5p served as the target of circSLIT2 in PDAC cells.

From: Novel circular RNA circSLIT2 facilitates the aerobic glycolysis of pancreatic ductal adenocarcinoma via miR-510-5p/c-Myc/LDHA axis

Fig. 4

A Subcellular fraction analysis indicated the cytoplasmic or nuclear distribution of circSLIT2. B Expression of miRNAs predicted by bioinformatics analysis (https://circinteractome.nia.nih.gov/) using RT-qPCR in PANC-1 cells transfected with circSLIT2 knockdown. C RNA fluorescence in situ hybridization (RNA-FISH) unveiled the distribution of miR-510-5p (labeled by FAM probe) and circSLIT2 (labeled by Cy3 probe). D RNA immunoprecipitation (RIP) assay was performed in PANC-1 cells to confirm the association between circSLIT2 and miR-510-5p. E The wild type (WT) correspond with miR-510-5p and mutant (Mut) vector for circSLIT2 were constructed. Luciferase reporter assay indicated the covalent binding within miR-510-5p and circSLIT2 wild type (WT) or mutant (Mut) vector. *p < 0.05; **p < 0.01.

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