Fig. 5: c-Myc served as a direct target of miR-510-5p and promoted LDHA transcription. | Cell Death & Disease

Fig. 5: c-Myc served as a direct target of miR-510-5p and promoted LDHA transcription.

From: Novel circular RNA circSLIT2 facilitates the aerobic glycolysis of pancreatic ductal adenocarcinoma via miR-510-5p/c-Myc/LDHA axis

Fig. 5

A miR-510-5p harbored the covalent complementary binding sites with c-Myc mRNA at 3′-UTR. B Luciferase reporter analysis unveiled the combination within miR-510-5p mimics and c-Myc mRNA wild type (WT) or mutant (Mut) sequences at 3′-UTR. C RT-qPCR showed the expression of c-Myc mRNA level in Capan-2 and PANC-1 cells administrated with miR-510-5p mimics transfection. D RT-qPCR showed the expression of c-Myc mRNA level in Capan-2 and PANC-1 cells administrated with circSLIT2 overexpression. E Schematic diagram illustrated the binding sites of transcription factor c-Myc at the promoter region of LDHA near transcriptional start site (TSS). F Chromatin immunoprecipitation (ChIP) elucidated the enrichment of LDHA promoter binding sites sequence in the precipitated complex with c-Myc antibody. G Western blot analysis uncovered the LDHA protein level after c-Myc overexpression transfection. H Luciferase reporter vectors for LDHA promoter binding sites, including wild type (WT) and mutant (Mut) sequences, were synthesized. I Luciferase reporter analysis elucidated the interaction of c-Myc and LDHA promoter region. **p < 0.01.

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