Fig. 3: SRXN1 attenuates AP by inducing oxidative stress and apoptosis. | Cell Death & Disease

Fig. 3: SRXN1 attenuates AP by inducing oxidative stress and apoptosis.

From: Sulfiredoxin-1 attenuates injury and inflammation in acute pancreatitis through the ROS/ER stress/Cathepsin B axis

Fig. 3

A J14 (5 μM) significantly increased the DCF signal induced by cerulein (100 nM) in isolated acini. B J14 increased the MDA concentration and decreased SOD activity upon cerulein treatment in mice. C The cleaved caspase 3 level was substantially induced by cerulein (100 nM), and cotreatment with J14 further augmented it in acinar cells. D, E The cleaved caspase 3 was induced by cerulein at a dose of 50 µg/kg body weight eight times, and injection of J14 further promoted it in mice; TUNEL assay showed the same trend. F Acinar cells were isolated and cultured for 3 days, and Srxn1 siRNA was transfected for another 3 days and then cerulein (100 nM) was added. Amylase served as an indicator of acinar cells. G, H Lentivirus vector (mCherry) was transfected to overexpress Srxn1; IF staining of cleaved caspase 3 showed that Srxn1 overexpression prevented apoptosis induced by cerulein in acini. N. S no significance; Con control; NC negative control; OE overexpression; *p < 0.05, **p < 0.01, ***p < 0.001. Data represent three or more experiments for in vitro assays and eight for in vivo assays.

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