Fig. 4: LCL-161 selectively induces TNF mediated apoptosis of HBV infected primary human liver organoids. | Cell Death & Disease

Fig. 4: LCL-161 selectively induces TNF mediated apoptosis of HBV infected primary human liver organoids.

From: Clinical stage drugs targeting inhibitor of apoptosis proteins purge episomal Hepatitis B viral genome in preclinical models

Fig. 4

A HBV DNA levels in supernatant of HBV-infected liver organoid cultures 4 days after indicated treatment with 10 μM LCL-161, 200 ng/ml recombinant TNF or the combinaton of both (8 days after infection), identical donor derived organoid cultures were used in all groups (n = 3 for LCL-161 only group, n = 5 for all other groups). Data are represented as mean ± s.e.m. and normalized to untreated organoid culture. Statistical analyses using unpaired two-tailed t tests for parametric data was performed. **P < 0.01. B Immunofluorescence staining of differentiated liver organoids, uninfected or infected with HBV and treated with 10 μM LCL-161 and 200 ng/ml TNF for 6 h on day 6 post infection. Scale bars: 50 μm. Donor-derived organoid cultures with n = 2 for uninfected and n = 4 for uninfected groups were used. DIC differential interference contrast, cC3 cleaved caspase 3. Results are representative from three independent experiments.

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