Fig. 7: H19 contributes to the activation and polarization of macrophages in the cholestatic livers.

A Representative images of co-staining of CD31 and CD11b, α-SMA and Ccr2, F4/80 and CK19 in the liver sections from Sham, Sham + TAM, BDL, and BDL + TAM mice (each group, n = 3–6). Scale bars: 25 μm. B Quantification of CD11b+, Ccr2+, and F4/80+ cells of panel A. C Western blotting analysis for CD11b, iNOS, Ccr2, and β-actin in the liver tissues from Sham, Sham + TAM, BDL, and BDL + TAM mice. D Quantification of panel B. Data were expressed as mean ± SD. *P < 0.05; **P < 0.01, ***P < 0.001.