Fig. 5: Comparison of the C26 model with integrative data and gene validation.

A The Venn plot shows the overlap of 127 upregulated DEGs and 126 downregulated ‘DEGs in the C26 model with merged data. B The top 25 predominately enriched pathways of upregulated and downregulated genes in the merged dataset and C26 dataset were compared. C Validation of the upregulated and downregulated DEGs in the enriched KEGG pathways in the merged dataset was performed by qPCR. Data are expressed as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, N = 10.