Fig. 1: Ad-delE1B-mediated growth inhibitory effects were independent of p53 expression. | Cell Death & Disease

Fig. 1: Ad-delE1B-mediated growth inhibitory effects were independent of p53 expression.

From: An MDM2 inhibitor achieves synergistic cytotoxic effects with adenoviruses lacking E1B55kDa gene on mesothelioma with the wild-type p53 through augmenting NFI expression

Fig. 1

A Growth inhibitory effects of Ad-delE1B. Mesothelioma with the wild-type p53 (MSTO-211H, NCI-H226 and NCI-H28) or mutated p53 genotype (EHMES-1 and JMN-1B) were treated with Ad-delE1B or Ad-LacZ at various vp for 4 days. The cell viabilities were measured with the WST agent and relative viability was calculated based on untreated cells. IC50 values (vp/cell) were calculated with GraphPad Prism software. Averages and SE bars are shown (n = 3). B Cells were treated with Ad-delE1B (3 × 103 vp/cell) and cultured for 2 or 4 days. Expression of p53, phosphorylated p53 at Ser 15 (p-P53 S15), and actin as a control was examined with Western blot analysis. C Cells transfected with p53 siRNA or with non-target control siRNA (Ctrl siRNA) were treated with Ad-delE1B (3 × 103 vp/cell) for 4 days. Expression of p53 and actin as a control was examined with Western blot analysis. Cells transfected with siRNA were also treated with Ad-delE1B at various vp for 4 days and the cell viabilities were measured with the WST agent. Relative viability was calculated based on untreated cells. Averages and SE bars are shown (n = 3).

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