Fig. 2: MDM2 inhibitors increased p53 expression in wild-type p53 mesothelioma and the growth inhibition was relevant to p53 genotype. | Cell Death & Disease

Fig. 2: MDM2 inhibitors increased p53 expression in wild-type p53 mesothelioma and the growth inhibition was relevant to p53 genotype.

From: An MDM2 inhibitor achieves synergistic cytotoxic effects with adenoviruses lacking E1B55kDa gene on mesothelioma with the wild-type p53 through augmenting NFI expression

Fig. 2

A Cells were treated with various concentrations of nutlin-3a for 4 days or RG7112 for 3 days. The cell viabilities were measured with the WST agent and relative viability was calculated based on untreated cells. Averages and SE bars are shown (n = 3). B Cells were treated with various concentrations of nutlin-3a or RG7112 as indicated for 2 days. Expression of p53 and phosphorylated p53 at Ser 15, and actin as a control was examined with western blot analysis. C Cells transfected with p53 siRNA or control siRNA were treated with various concentrations of nutlin-3a or RG7112 for 3 days and the cell viabilities were measured with the WST agent. Relative viability was calculated based on untreated cells. Averages and SE bars are shown (n = 3).

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