Fig. 4: Combination of Ad-delE1B and MDM2 inhibitors augmented apoptotic pathways and DNA damage signals. | Cell Death & Disease

Fig. 4: Combination of Ad-delE1B and MDM2 inhibitors augmented apoptotic pathways and DNA damage signals.

From: An MDM2 inhibitor achieves synergistic cytotoxic effects with adenoviruses lacking E1B55kDa gene on mesothelioma with the wild-type p53 through augmenting NFI expression

Fig. 4

A Cells were infected with Ad-delE1B or Ad-LacZ (3 × 103 vp/cell) for 2 days, and then treated with nutlin-3a (15 µM) or RG7112 (10 µM) for further 16 h in MSTO-211H or 36 h in NCI-H226. Cell lysate of wild-type (A) or mutated p53 cells (B) were subjected to western blot analysis and expression levels of molecules associated with p53 (A, B), apoptosis (A), autophagy (C), and DNA damage pathways (D) were examined with respective Ab as indicated.

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