Fig. 6: Enhanced NFI expression with MDM2 inhibitors. | Cell Death & Disease

Fig. 6: Enhanced NFI expression with MDM2 inhibitors.

From: An MDM2 inhibitor achieves synergistic cytotoxic effects with adenoviruses lacking E1B55kDa gene on mesothelioma with the wild-type p53 through augmenting NFI expression

Fig. 6

Cells were infected with Ad-delE1B or Ad-LacZ (3 × 103 vp/cell) for 2 days, and then treated with nutlin-3a (15 µM) or RG7112 (10 µM) for further 16 h in MSTO-211H or 36 h in NCI-H226. A Cell lysate was examined for expression of cellular factors linked with Ad replications with Western blot analysis. NFI: An upper band ubiquitously expressed was non-specific and a lower band corresponding to 55 kDa was authentic. B–D Cells were stained with Ab against NFI or p53, or with DAPI or PI, and the images were analyzed with a laser confocal microscope. C Expression of p53 was knocked down with p53 siRNA and non-target siRNA was used as a control. Cells transfected with siRNA were infected with Ad-delE1B for 2 days, and then treated with nutlin-3a (15 µM) or RG7112 (10 µM) for further 16 h in MSTO-211H or 36 h in NCI-H226. D Cells were infected with several vp of Ad-p53 as indicated for 2 days. E A model of Ad-delE1B and p53 upregulated by MDM2 inhibitors in Ad-mediated cytotoxicity and virus replications. Scale bar: 10 μm.

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