Fig. 3: USP22 promotes CCA tumorigenesis and metastasis in vivo. | Cell Death & Disease

Fig. 3: USP22 promotes CCA tumorigenesis and metastasis in vivo.

From: Operative ubiquitin-specific protease 22 deubiquitination confers a more invasive phenotype to cholangiocarcinoma

Fig. 3

A–F Each of 1 × 107 HCCC-USP22, HCCC-EV, RBE-shUSP22 or RBE-shControl cells were subcutaneously injected into the flanks of 5-week-old nude mice. Tumour volume was monitored once a week; when they reached the maximum permitted volumes, mice were sacrificed, and tumours were weighted (*p < 0.05, n = 5). G–H Each of 1 × 107 HCCC-USP22, HCCC-EV, RBE-shUSP22 or RBE-shControl cells were injected into the tail veins of nude mice. After 6 weeks, animals were sacrificed, and their lungs were harvested and fixed for H&E staining. Numbers of metastatic foci were quantified. Data are shown as the mean ± SD (*p < 0.05, n = 8). Representative images of metastatic foci in sections of the lungs.

Back to article page