Fig. 3: RSPO2 inhibited the apoptosis of porcine ovarian GCs.
From: DNA methylation mediated RSPO2 to promote follicular development in mammals
A–C The mRNA (A) and protein (B) expression levels of Caspase3, Caspase8, Caspase9, BAX, and BCL2, as well as the cleaved protein (C) expression levels of Caspase3, Caspase8, Caspase9, and PARP in porcine GCs with RSPO2 overexpression. D The apoptosis rates of GCs with RSPO2 overexpression was assessed by flow cytometry. E–G The mRNA (E) and protein (F) expression levels of Caspase3, Caspase8, Caspase9, BAX, and BCL2, as well as the cleaved protein (G) expression levels of Caspase3, Caspase8, Caspase9, and PARP in porcine GCs with RSPO2 knockdown. H The apoptosis rates of GCs with RSPO2 knockdown was assessed by flow cytometry. I The mRNA expression levels of Caspase3, Caspase8, Caspase9, BAX, and BCL2 in porcine GCs transfected with RSPO2-siRNA5. J The apoptosis rates of GCs transfected with RSPO2-siRNA5 was assessed by flow cytometry. *P < 0.05, **P < 0.01.
