Fig. 5: RSPO2 knockdown blocked the development of porcine follicles.
From: DNA methylation mediated RSPO2 to promote follicular development in mammals
A, B The mRNA (A) and protein (B) expression levels of RSPO2, Caspase3, PCNA, CCND1, CYP19A1, LGR4, and CTNNB1 in porcine follicle with RSPO2 inhibition. The numbers below bands were folds of band intensities relative to control. The band intensities were measured by ImageJ and normalized to Tubulin. C The photos of porcine follicles cultured in vitro after transfected with sh-RSPO2 on first, fourth, and seventh day. D The apoptosis of follicular GCs was detected by TUNEL after transfected with sh-RSPO2 at the fourth day. *P < 0.05, **P < 0.01.
