Table 1 Primers used for qRT-PCR in pig.
From: DNA methylation mediated RSPO2 to promote follicular development in mammals
Gene name | Primer sequences (5’ → 3’) | Size (bp) | Accession number |
|---|---|---|---|
RSPO2 | F: GATGGAGACGCAGTAAGCGA R: CATATCTGGGGCTCGGTGTC | 197 | NM_001293141.1 |
Caspase3 | F: ACATGGAAGCAAATCAATGGAC R: TGCAGCATCCACATCTGTACC | 154 | NM_214131.1 |
Caspase8 | F: GAGCCTGGACTACATCCCAC R: GTCCTTCAATTCCGACCTGG | 283 | NM_001031779.2 |
Caspase9 | F: GCTGAACCGTGAGCTTTTCA R: CCTGGCCTGTGTCCTCTAAG | 161 | XM_003127618.4 |
BAX | F: ACTTCCTTCGAGATCGGCTG R: AAAGACACAGTCCAAGGCGG | 184 | XM_013998624.2 |
BCL2 | F: GATGCCTTTGTGGAGCTGTATG R: CCCGTGGACTTCACTTATGG | 145 | XM_021099593.1 |
PCNA | F: TCGTTGTGATTCCACCACCAT R:TGTCTTCATTGCCAGCACATT | 278 | NM_001291925.1 |
CDK1 | F: AGGTCAAGTGGTAGCCATGAA R: CATGAACTGACCAGGAGG | 225 | NM_001159304.2 |
CDK2 | F: AAAGATCGGAGAGGGCACG R: GCAGTACTGGGTACACCCTC | 121 | NM_001285465.1 |
CDK4 | F: CCTCCCGGTATGAACCAGTG R: TGCTCAAACACCAGGGTCAC | 277 | NM_001123097.1 |
CCNA1 | F: GCGCCAAGGCTGGAATCTAT R: CCTCAGTCTCCACAGGCTAC | 196 | XM_005668339.3 |
CCNA2 | F: GTACTGAAGGCCGGGAACTC R: AGCTGGCCTCTTTTGAGTCT | 192 | NM_001177926.1 |
CCNB1 | F: ACGGCTGTTAGCTAGTGGTG R: GAGCAGTTCTTGGCCTCAGT | 236 | NM_001170768.1 |
CCNB2 | F: TGGAAATCGAGTTACAACCAGA R: TGGAGCCAACATTTCCATCTGT | 151 | NM_001114282.1 |
CCND1 | F: CTTCCATGCGGAAGATCGTG R: GGAGTTGTCGGTGTAGATGC | 234 | XM_021082686.1 |
CCND2 | F: TTCCCCAGTGCTCCTACTTC R: CACAACTTCTCAGCCGTCAG | 259 | NM_214088.1 |
CCNE1 | F: AGCCTGTGAAAACCCCTGTT R: TCCAGAAGAATCGCTCGCAT | 252 | XM_005653265.2 |
CCNE2 | F: GGGGGATCAGTCCTTGCATT R: AGCCAAACATCCTGTGAGCA | 154 | NM_001243931.1 |
CYP19A1 | F: CTGAAGTTGTGCCTTTTGCCA R: CTGAGGTAGGAAATTAGGGGC | 139 | NM_214429.1 |
STAR | F: CGACGTTTAAGCTGTGTGCT R: ATCCATGACCCTGAGGTTGGA | 136 | NM_213755.2 |
HSD17B1 | F: GTCTGGCATCTGACCCATCTC R: CGGGCATCCGCTATTGAATC | 166 | NM_001128472.1 |
HSD3B1 | F: ATCTGCAGGAGATCCGGGTA R: CCTTCATGACGGTCTCTCGC | 216 | NM_001004049.2 |
E2F1 | F: GACTCCTCGCAGATCGTCAT R: CGCCTCCAGGCCAAACATAG | 263 | XM_021077692.1 |
LGR4 | F: GACCGTCGGGTAGATTGCTC R: GCTCCTCTAGAAAAGGGAAGTT | 145 | XM_021083233.1 |
CTNNB1 | F: AAGCGGCTGTTAGTCACTGG R: TGAGCGCGAGTCATTGCATA | 220 | NM_214367.1 |
GAPDH | F: TCACCAGGGCTGCTTTTAACT R: CTTGACTGTGCCGTGGAACT | 131 | NM_001206359.1 |
