Table 3 Primers used for vector construction.
From: DNA methylation mediated RSPO2 to promote follicular development in mammals
Primer name | Primer sequences (5’ → 3’) | Size (bp) |
|---|---|---|
pcDNA3.1-RSPO2 | F: CCAAGCTTTCCTTTGCCCTCATCATCCTG R: GGGGTACCAGCTAGGAAGACGCTGTGTTG | 693 |
pcDNA3.1-E2F1 | F: CCAAGCTTCCCCATCCTGCGATTGGC R: GGGGTACCCCCATCAGAAATCCAGGGGG | 1511 |
pGL3-RSPO2 | F: CGACGCGTACCTCAGGGGCTGAATTTTCTT R: CCCTCGAGTTGGCAGTGGCTGTAATCCAT | 2299 |
pGL3-NCGI1 | F: CGACGCGTGGATAAGCAAAGGATTAGAATA R: CCCTCGAGCCAGGCTCTGACTGCTGCCTCT | 183 |
pGL3-CGI1 | F: CGACGCGTAAGGTCTAGGAGAAACCATCAG R: CCCTCGAGTGAGGAGTAGCTGAGGTGG | 180 |
pGL3-NCGI2 | F: CGACGCGTACCTCAGCTACTCCTCAATTTA R: CCCTCGAGGTCCAAGGCAACTAAGTCAG | 184 |
pGL3-CGI2-1 | F: CGACGCGTTTGGACCACAGCACCACCTAC R: CCCTCGAGCAGCGCCTAGCTGGAGCGCAGT | 293 |
pGL3-CGI2-2 | F: CGACGCGTCTCCGCGCACTTCGAAACCACT R: CCCTCGAGGACCCATAGGCTCCGACCCGG | 347 |
pGL3-NCGI3 | F: CGACGCGTCTCCAAGTTGCCGGCAGCTG R: CCCTCGAGCTGCGGTGCAAGACTCAGAGG | 251 |
