Fig. 4: AC073352.1 binds to the transcriptional activator YBX1. | Cell Death & Disease

Fig. 4: AC073352.1 binds to the transcriptional activator YBX1.

From: A novel long non-coding RNA AC073352.1 promotes metastasis and angiogenesis via interacting with YBX1 in breast cancer

Fig. 4

a AC073352.1 expression in MDA-MB-231 and MDA-MB-468 cells was detected by RNA FISH. Scale bars: 20 μm. b AC073352.1 sense and antisense RNAs were used for in vitro transcribed and pull-down assays in MDA-MB-231 cells. After electrophoresis and silver staining, the different bans located at 45–60 kDa. c Western blot analysis of the specific association between YBX1 and biotinylated AC073352.1; results obtained from three independent streptavidin RNA pull-down assays. d RIP assays were performed using an anti-YBX1 antibody; lgG was used as negative control. Specific primers were used to detect AC073352.1 (n = 3). e The co-localization of AC073352.1 and YBX1 was assessed by FISH and immunofluorescence. Scale bar: 10 μm. ***p < 0.001.

Back to article page