Fig. 7: Exosomal AC073352.1 regulates the angiogenesis of HUVECs via binding YBX1. | Cell Death & Disease

Fig. 7: Exosomal AC073352.1 regulates the angiogenesis of HUVECs via binding YBX1.

From: A novel long non-coding RNA AC073352.1 promotes metastasis and angiogenesis via interacting with YBX1 in breast cancer

Fig. 7

a Flow chart for the exosome purification procedure based on differential ultracentrifugation. b TEM scanning images of purified exosomes derived from MDA-MB-231 cells overexpressing AC073352.1. Scale bars: 100 nm. c Characterization of purified exosomes derived from MDA-MB-231 cells overexpressing AC073352.1. The size, number, and distribution of exosomes was analyzed by NTA. d Exosomal protein marker (CD63 and CD9) detection by western blot from purified exosomes and exosome-depleted cell extracts. e qRT-PCR analysis of AC073352.1 levels in exosomes from AC073352.1-overexpressing MDA-MB-231 cells and shAC073352.1-treated MDA-MB-468 cells. f qRT-PCR analysis of AC073352.1 levels: AC073352.1 levels were decreased in exosomes after MDA-MB-231 cells were transfected with a YBX1 siRNA. g HUVECs were incubated with PKH67-lableled exosomes for intercellular trafficking. Scale bars: 10 μm. h, i Representative images and quantification of tube-formation assays by HUVECs treated with MDA-MB-231vector or MDA-MB-231AC073352.1. Scale bars: 100 μm. *p < 0.05 and **p < 0.01.

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