Fig. 2: The impact of TRB3 on the phosphorylation of AKT registered in MIO-M1 cells overexpressing TRB3.
From: Tribbles homolog 3-mediated targeting the AKT/mTOR axis in mice with retinal degeneration
A MIO-M1 cells were transfected with AAV-CAG-TRB3-GFP plasmid. Seventy-two hours post-transfection, the cells were harvested, and protein extracts were separated running polyacrylamide gel. Images of western blots stained with anti-TRB3 and anti-pAKTSer473 antibodies are shown. B Quantitation of p-AKT normalized to AKT is shown. About a 50% reduction in p-AKT level was observed (n = 4). C MIO-M1 cells were transfected with AAV-CAG-TRB3-GFP plasmid in addition to control and anti-TRB3 ASOs for 48 h. TRB3, p-AKT, and AKT were detected in the protein extracts. D An analysis of densitometry of TRB3 and pAKTSer473 bands normalized to the bands for β-actin and AKT, respectively, is shown (n = 4). Data are shown as mean ± SEM; a.u. = arbitrary units; **p < 0.01, ***p < 0.001.
