Fig. 4: The exploration and verification of downstream underlying ALPK2 induced regulation of bladder cancer.
From: ALPK2 acts as tumor promotor in development of bladder cancer through targeting DEPDC1A
A A PrimeView Human Gene Expression Array was performed to identify the differentially expressed genes (DEGs) between shALPK2 and shCtrl groups of T24 cells. qPCR (B) and western blotting (C) were used to detect the expression of several selected DEGs in T24 cells with or without ALPK2. D The expression of DEPDC1A in bladder cancer tissues and normal tissues was evaluated by IHC analysis (scale bar = 50 μm in 200 magnification, scale bar = 20 μm in 400 magnification). E The mRNA and protein expression of ALPK2 in EJ, T24, J82, and RT4 cell lines was detected by qPCR and western blot, respectively. F The interaction between ALPK2 protein and DEPDC1A protein was confirmed by co-immunoprecipitation assay. The representative images were selected from at least three independent experiments. Data were shown as mean ± SD. *P < 0.05.
