Fig. 1: Role of ZFP36 in intestinal ischemia–reperfusion (I/R)-induced acute lung injury. | Cell Death & Disease

Fig. 1: Role of ZFP36 in intestinal ischemia–reperfusion (I/R)-induced acute lung injury.

From: ZFP36 protects lungs from intestinal I/R-induced injury and fibrosis through the CREBBP/p53/p21/Bax pathway

Fig. 1

C57BL/6 mice were subjected to 60 min of intestinal ischemia followed by 0, 30, 60, and 90 min of reperfusion as indicated. Sham mice were included as the control. A ZFP36 mRNA and protein expression in lung tissues were analyzed by RT-qPCR and Western blot (n = 6 per group, **P < 0.01). B Immunohistochemical staining of lung tissues for ZFP36 of sham and I/R 60 min. Scale bars: 50 μm. C–F Levels of IL-1β (C), TNF-α (D), and IL-6 (E) were measured by ELISA and Western blotting (F). G H&E staining of lung tissues. Red arrows outline collapsed alveoli, blue arrows outline multiple inflammatory cells infilitration, black arrows outline bronchial hemorrhage. Scale bars: 50 μm. H–J Arterial blood PaO2 (H), lung water content (I), and BALF protein content (J) were measured.

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