Fig. 4: lncRNA RCAT1 knockdown suppresses malignant phenotypes in RCC cells. | Cell Death & Disease

Fig. 4: lncRNA RCAT1 knockdown suppresses malignant phenotypes in RCC cells.

From: LncRNA RCAT1 promotes tumor progression and metastasis via miR-214-5p/E2F2 axis in renal cell carcinoma

Fig. 4

a The lncRNA RCAT1 mRNA levels in 786-O and 769-P cells transfected with lncRNA RCAT1 or negative control siRNAs. b The cell proliferation of 786-O and 769-P cells in response to lncRNA RCAT1 knockdown was measured using MTT assay. c Colony-formation assays performed with the 786-O and 769-P cells transfected with lncRNA RCAT1 or negative control siRNAs. d EdU assay was used to evaluate the effect of lncRNA RCAT1 knockdown on cell proliferation. Scale bar, 200 μm. e Apoptosis was assayed by flow cytometry in 786-O and 769-P cells after lncRNA RCAT1 knockdown. f The wound-healing assay was performed to examine the migration abilities after lncRNA RCAT1 knockdown. Scale bar, 200 μm. g The effects of lncRNA RCAT1 knockdown on migration and invasive abilities of 786-O and 769-P cells were evaluated by the transwell assays. Scale bar, 200 μm (*P < 0.05, **P < 0.01, ***P < 0.001).

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