Fig. 5: MiR-214-5p was one target of lncRNA RCAT1. | Cell Death & Disease

Fig. 5: MiR-214-5p was one target of lncRNA RCAT1.

From: LncRNA RCAT1 promotes tumor progression and metastasis via miR-214-5p/E2F2 axis in renal cell carcinoma

Fig. 5

a The subcellular location of lncRNA RCAT1 in 786-O cells. U6 and GAPDH were used as internal controls. b FISH assay was used to detect the subcellular location of lncRNA RCAT1 in 786-O cells. Scale bar, 2000 μm. c The lncRNA RCAT1-centric ceRNA network. d Predicted binding sites of miR-214-5p in lncRNA RCAT1 sequence. e Dual-luciferase assay was performed to confirm the interaction between miR-214-5p and lncRNA RCAT1. f The expression levels of lncRNA RCAT1 and miR-214-5p were detected in the substrate of RIP assay by qRT-PCR. g lncRNA RCAT1 knockdown led to increased expression of miR-214-5p (*P < 0.05, **P < 0.01, ***P < 0.001).

Back to article page