Fig. 7: lncRNA RCAT1 promoted RCC cell progression through protecting E2F2 from miR-214-5p-induced degradation. | Cell Death & Disease

Fig. 7: lncRNA RCAT1 promoted RCC cell progression through protecting E2F2 from miR-214-5p-induced degradation.

From: LncRNA RCAT1 promotes tumor progression and metastasis via miR-214-5p/E2F2 axis in renal cell carcinoma

Fig. 7

a The differential expression of E2F2 in ccRCC tissues and normal tissues based on TCGA and GEO databases. b The differential expression of E2F2 in ccRCC tissues with or without metastasis. c The Kaplan–Meier analysis was used to evaluate the relationship between E2F2 expression and overall survival time of ccRCC patients. d, e Overexpression of miR-214-5p (d) or lncRNA RCAT1 (e) knockdown led to decreased mRNA and protein levels of E2F2 in RCC cells. f Overexpression of miR-214-5p effectively reverses lncRNA RCAT1-induced increased mRNA and protein levels of E2F2. g The schematic illustration showing the predicted binding sites of miR-214-5p in 3′UTR of E2F2. h Luciferase assay was used to show the regulatory relationship between miR-214-5p and E2F2. i The efficiency of E2F2 knockdown was detected by qRT-PCR and western blot. j Cell proliferation was evaluated after E2F2 knockdown using MTT assay. k Cell apoptosis was evaluated after E2F2 knockdown using flow cytometry assay. l Cells migration and invasion abilities were detected after E2F2 knockdown by transwell assays. Scale bar, 200 μm (**P < 0.01, ***P < 0.001).

Back to article page