Fig. 5: MiR-195 knockdown upregulates Smurf2 to inhibit YY1 and represses HG-induced EMT and cell permeability of ARPE-19 cells. | Cell Death & Disease

Fig. 5: MiR-195 knockdown upregulates Smurf2 to inhibit YY1 and represses HG-induced EMT and cell permeability of ARPE-19 cells.

From: MiR-195 inhibits the ubiquitination and degradation of YY1 by Smurf2, and induces EMT and cell permeability of retinal pigment epithelial cells

Fig. 5

ARPE-19 cells were transfected with inhibitor NC or miR-195 inhibitor, and were then stimulated or not stimulated by HG. A MiR-195 abundances were measured via qRT-PCR in the transfected cells. B Smurf2 and YY1 abundances were measured via western blotting in the transfected cells. C, D Cell migration was measured using wound-healing analysis and transwell assay in the treated cells. E Occludin, E-cadherin, and Vimentin abundances were determined by immunofluorescence in the treated cells. F Cell permeability was evaluated via FITC-dextran assay in the treated cells. G VEGFA, Snail1, Occludin, E-cadherin, N-cadherin, and Vimentin levels were detected via western blotting in the treated cells. For each analysis, three technical replicates were performed and three biologically independently performed replicates are included, *p < 0.05, **p < 0.01, ***p < 0.001.

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