Fig. 6: HMGB2 binds to the promoter of C/EBPβ in MCE stage to modulate adipogenesis. | Cell Death & Disease

Fig. 6: HMGB2 binds to the promoter of C/EBPβ in MCE stage to modulate adipogenesis.

From: HMGB2 orchestrates mitotic clonal expansion by binding to the promoter of C/EBPβ to facilitate adipogenesis

Fig. 6

a ChIP-seq analysis of the peak distributions of C/EBPβ, PPARγ, and FABP4 loci bound by HMGB2 at −48, 24, and 96 h. b ChIP-PCR assay for the interaction between HMGB2 and C/EBPβ promoter in 3T3-L1 cells. c Band intensity for b (n = 3, *P < 0.05). d Luciferase activity for truncated DNA fragments of C/EBPβ (***P < 0.001). Experiments were conducted in 293T cells. e Binding motif of HMGB2 on C/EBPβ. HOMER and KNOWN analysis by ChIP-seq on FDR-corrected, p values represent enrichment. f Luciferase activity of potential regions of C/EBPβ bound by HMGB2 before and after mutation (***P < 0.001). Experiments were carried out in 293T cells. g Oil Red-O staining assay in 3T3-L1 cells (left) and OD measurements (right). h Western blot for adipogenic genes. (g, h: 3T3-L1 cells transfected with si-NC, si-HMGB2, and si-HMGB2 with C/EBPβ overexpression plasmid, respectively).

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