Fig. 2: Silencing of PSMC2 inhibits breast cancer development in vitro.

A MTT assay showed that knocking down the expression of PSMC2 in breast cancer cells inhibited cell proliferation. B Flow cytometry suggested that PSMC2 knockdown promoted apoptosis of breast cancer cells. C The detection of cell cycle distribution indicated that PSMC2 knockdown induces cell cycle arrest in G2 phase. D Transwell assay displayed the weakened migration ability of breast cancer cells after silencing PSMC2. E The expression of EMT markers including N-cadherin, Snail and Vimentin was decreased in PSMC2 knockdown cells. Data were shown as mean ± SD (n ≥ 3). * P < 0.05, *** P < 0.001.