Fig. 4: In murine and human osteoblasts iKeap1 ameliorates dexamethasone- and nicotine-induced oxidative injury and cell death. | Cell Death & Disease

Fig. 4: In murine and human osteoblasts iKeap1 ameliorates dexamethasone- and nicotine-induced oxidative injury and cell death.

From: A novel Keap1 inhibitor iKeap1 activates Nrf2 signaling and ameliorates hydrogen peroxide-induced oxidative injury and apoptosis in osteoblasts

Fig. 4

The primary murine osteoblasts (A–F) or the primary human osteoblasts (G–J) were pretreated (for 2 h) with iKeap1 (10 μM), followed with or without dexamethasone (DEX, 2 μM)/nicotine (1 μM) stimulation, cells were further cultured for applied time periods, ROS production, cell viability, apoptosis, and necrosis were tested by CellROX staining (A, B and F), CCK-8 (C and G), nuclear TUNEL staining (D and H), and medium LDH release (E and I) assays, respectively, and results were normalized and quantified. Quantified values were mean ± standard deviation (SD, n = 5). “Veh” stands for the vehicle control (0.1% DMSO). *P < 0.05. Experiments were repeated five times, with similar results obtained. Scale bar = 100 μm (A).

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