Fig. 1: MYBL2 expression was upregulated in parallel with RRM2 level in clinical CRC patient samples. | Cell Death & Disease

Fig. 1: MYBL2 expression was upregulated in parallel with RRM2 level in clinical CRC patient samples.

From: A MYBL2 complex for RRM2 transactivation and the synthetic effect of MYBL2 knockdown with WEE1 inhibition against colorectal cancer

Fig. 1

A The mRNA expression profiles (left) and the fold changes (right) of RRM1, RRM2, and RRM2B in CRC. All mRNA expression data of three RR subunit genes in CRC were downloaded from Oncomine database. DEGs were screened by “data type = mRNA, concept filter = cancers vs. normal, P < 0.05.” The y-axis of left panel presented the number of studies in which RRM1, RRM2, and RRM2B were differentially expressed according to the criteria. Fold changes shown in the color cells of the right panel were calculated by “cancer vs. normal” from the differentially expressed studies in the left panel. Dark red represented cancers in which the interested gene had a gene rank of the top 1% in the elevated-expression of the DEGs, and red and brick red marked top 5%, or 10%, respectively, while dark blue, blue, and light blue colors marked the top 1, 5, and 10% in the decreased-expression of the DEGs, respectively. Gene rank: genes are ranked by the p-value list. B, C The increased mRNA expression of RRM2 during CRC development. The mRNA expression data and clinicopathological information of CRC patients were downloaded from GSE20916 (B) and TCGA (C), respectively. D Screening of RRM2-correlated transcription factors (TFs). The expression correlation between RRM2 and all other genes in four CRC cohorts was measured by Pearson correlation coefficient. The human TFs that were positively correlated with RRM2 expression (R > 0.5, p < 0.05) were selected and listed in alphabetical order. E, F The increased mRNA expression of MYBL2 during CRC development. The mRNA expression data and clinicopathological information of CRC patients were downloaded from GSE20916 (E) and TCGA (F), respectively. G The expression correlation between MYBL2 and RRM2 in colorectal normal, adenoma, and carcinoma tissues, respectively. The mRNA expression data and clinicopathological information of CRC patients were downloaded from GEO (GSE20916, GSE8671, and GSE35896). H The protein expression level of MYBL2 and RRM2 determined by IHC in the paired cancer and adjacent normal tissues from 69 CRC patients. I Representative IHC images of MYBL2 and RRM2 in the CRC and adjacent noncancerous tissues (consecutive sections). Scale bars: 200 μm (100×), 25 μm (400×). J The correlation of IHC scores between MYBL2 and RRM2 in the 69 CRC tissues. ns, not signifucant; *p < 0.05; **p < 0.005, ***p < 0.001, ****p < 0.0001.

Back to article page