Fig. 1: Exosomes derived from WERI-Rb1 cells mediate the activity of HUVECs.

A Representative TEM images of WERI-Rb1 exosomes. The diameter of the vesicles was approximately 50–150 nm. Bar = 150 nm. B Nanoparticle tracking analysis (NTA) indicated that the average size and number of exosomes (97.7% of exosomes had a size of 108.0 nm). C Western blot analysis of the different protein markers (HSP70, CD9, TSG101, CD63) of exosomes collected from the WERI-Rb1 cells. D Exosomes labeled with PKH26 were swallowed by HUVECs (PKH26, red; CD31, green; DAPI, blue). Bar = 50 μm. E CCK-8 assays showed that the viability of the HUVECs was markedly elevated by the exosomes (n = 3, **P < 0.01). F Real-time PCR analysis of IL-1, IL-6, IL-8 and MCP-1 expression in the HUVECs incubated with the exosomes derived from WERI-Rb1 cells for 24 h. (n = 6, *P < 0.05, **P < 0.01, ***P < 0.001) according to two-tailed Student’s t test. G Real-time PCR analysis of VCAM1 and ICAM1 expression in the HUVECs incubated with the exosomes derived from WERI-Rb1 cells for 24 h (n = 6, *P < 0.05). H Western blot analysis indicating that exosome treatment upregulated VCAM1 and ICAM1 expression in the HUVECs. I Relative quantification of protein expression in HUVECs was performed by densitometry (n = 4, *P < 0.05).