Fig. 2: Exosomes derived from WERI-Rb1 cells induce HUVEC angiogenesis and migration in vitro.

A Wound-healing assay. HUVECs were treated with exosomes, and cell monolayers were scratched with 1–200-μl yellow tips. Images were captured at 0, 12, and 24 h after the wound scratch. B The rate of migration was measured by quantifying the total distance that the HUVECs moved from the edge of the scratch toward the center of the scratch. Relative quantification of HUVEC migration 24 h after the wound scratch (n = 7, ***P < 0.001). C Microscopic photographs of tubule formation on Matrigel are shown. There was a significant increase in HUVEC tubule connections in the presence of the exosomes compared with that in the presence of PBS. D The number of tubule junctions is presented in the histograms (n = 6, ***P < 0.001).