Fig. 4: JNK is required for the Hh activation and proliferation of colorectal cancer cells elicited by PGE2. | Cell Death & Disease

Fig. 4: JNK is required for the Hh activation and proliferation of colorectal cancer cells elicited by PGE2.

From: PGE2-JNK signaling axis non-canonically promotes Gli activation by protecting Gli2 from ubiquitin-proteasomal degradation

Fig. 4

A Luciferase assay for Gli transcriptional activity in colorectal cancer cells LS174T treated by PGE2, SP, TAT, JIP as indicated for 6 h. Error bars represent SD (n = 3). B The mRNA levels of Gli target genes in LS174T cells treated by PGE2, SP, TAT, JIP as indicated for 6 h were examined by real-time PCR and normalized to the mRNA level of gusb. Error bars represent SD (n = 3). C The proliferation of colorectal cancer cells LS174T treated by PGE2, SP,TAT, JIP as indicated for 6 h was examined with BrdU assays. Error bars represent SD (n = 3). D Luciferase assay for Gli transcriptional activity in colorectal cancer cells LS174T and SW480 cells expressing GFP, MKK7B2Jnk1a1, and MKK7B2Jnk1a1 (APF) after exposure to Gli inhibitors As2O3 (10 μM), GANT61 (20 μM), and JQ1 (1 μM) as indicated for 6 h. Error bars represent SD (n = 3). E The mRNA levels of Gli target genes in LS174T cells expressing GFP, MKK7B2Jnk1a1, and MKK7B2Jnk1a1 (APF) were examined by real-time PCR and normalized to the mRNA level of gusb after exposure to Gli inhibitors As2O3 (10 μM), GANT61 (20 μM), and JQ1 (1 μM) as indicated for 6 h. Error bars represent SD (n = 3). F The proliferation of colorectal cancer cells LS174T and SW480 cells expressing GFP, MKK7B2Jnk1a1, and MKK7B2Jnk1a1 (APF) was examined with BrdU assays after exposure to Gli inhibitors As2O3 (10 μM), GANT61 (20 μM), and JQ1 (1 μM) as indicated for 6 h. Error bars represent SD (n = 3). G Luciferase assay for Gli transcriptional activity in colorectal cancer cells LS174T expressing GFP, and Jnk1a1 (APF) after treated by PGE2 for 6 h. Error bars represent SD (n = 3). H The mRNA levels of Gli target genes in colorectal cancer cells LS174T expressing GFP, and Jnk1a1 (APF) after treated by PGE2 for 6 h were examined by real-time PCR and normalized to the mRNA level of gusb. Error bars represent SD (n = 3). I The proliferation of colorectal cancer cells LS174T expressing GFP, and Jnk1a1 (APF) after treated by PGE2 for 6 h was examined with BrdU assays. Error bars represent SD (n = 3). J Luciferase assay for Gli transcriptional activity in colorectal cancer cells LS174T cells expressing GFP, Gli2 WT, and Gli2T1546A after exposure to PGE2 as indicated for 6 h. Error bars represent SD (n = 3). K The proliferation of colorectal cancer cells LS174T cells expressing GFP, Gli2 WT, and Gli2T1546A after exposure to PGE2 was examined with BrdU assays. Error bars represent SD (n = 3). L Luciferase assay for Gli transcriptional activity in colorectal cancer cellsLS174T cells expressing GFP, Gli2 WT, and Gli2T1546E. Error bars represent SD (n = 3). M The proliferation of colorectal cancer cells LS174T cells expressing GFP, Gli2 WT, and Gli2T1546E was examined with BrdU assays. Error bars represent SD (n = 3).

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