Fig. 1: Schematic of functional screening by CRISPR/Cas9 SAM pooled library and irradiation treatment. | Cell Death & Disease

Fig. 1: Schematic of functional screening by CRISPR/Cas9 SAM pooled library and irradiation treatment.

From: Genome-wide CRISPR/Cas9 screening identifies CARHSP1 responsible for radiation resistance in glioblastoma

Fig. 1

A Schematic of irradiation-resistant GBM cells construction for high-throughput sequencing analysis. B U87 (0, 3, 6, 9, 12, 15 Gy/min) and U251 (0, 6, 9, 12, 15, 18 Gy/min) cell lines were treated with the indicated doses of metformin for 24 h, and cell viability was measured using an CCK8 assay. Data were represented as means ± SD of a representative experiment (of three experiments).

Back to article page