Fig. 2: PLEKHO2 deficiency sensitizes cells to TNFα/Smac mimetic induced apoptosis.
From: PLEKHO2 inhibits TNFα-induced cell death by suppressing RIPK1 activation
A WT and PLKEHO2−/− MEFs were treated with DMSO or TS (TNFα 20 ng/mL plus Smac mimetic 100 nM) for 8 h, cell viability was measured by ATP level. B WT and PLKEHO2−/− MEFs treated with DMSO or TS as A for 8 h. Cytotoxicity was measured by LDH release. C WT and PLKEHO2−/− MEFs were treated with TS as A for indicated periods of time. Cell lysates were probed with indicated antibodies. D WT and PLKEHO2−/− MEFs treated with DMSO or TS as A for 8 h, Caspase-3 activity was measured by Caspase-Glo 3 assay. Results are representative of at least three independent experiments.
