Fig. 4: PLEKHO2 promotes TNFα-induced NF-κB activation.
From: PLEKHO2 inhibits TNFα-induced cell death by suppressing RIPK1 activation
A, B WT and PLKEHO2−/− BMDMs (A) or Control and PLEKHO2 reexpressed PLKEHO2−/− MEFs (B) were treated with murine TNFα (20 ng/mL) for indicated periods of time, and cell lysates were probed with indicated antibodies. C mRNA expression of a panel of NF-κB targeted genes in WT or PLKEHO2−/− MEFs treated with TNFα (20 ng/mL) for indicated periods of time were determined by quantitative PCR (qPCR). D Reporter assay analysis of NF-κB activation in 293T cells expressing PLEKHO2 or not, treated with TNFα (20 ng/mL) or TNFα plus CHX. Results are representative of at least three independent experiments.
