Fig. 3: SMYD3 regulated the expression of S1PR1.
From: SMYD3 promotes hepatocellular carcinoma progression by methylating S1PR1 promoters
A Upper panel, the heat map of differentially expressed mRNAs in Huh7-SMYD3 cells compared with Huh7-Ctrl cells and in SK-Hep1-SMYD3 compared SK-Hep1-Ctrl cells (≥ 2-fold). The red color represents higher expression levels, and the green color represents lower expression levels (log2 fold change). Lower panel, 85 candidate genes were selected after determining the overlap of the two datasets. B Circos plots showing genes on each of the 23 chromosomes with increased SMYD3 occupancy in ChIP-seq (Huh7-SMYD3 red compared with Huh7-Ctrl blue). C Profile plot showing the distribution of indicated ChIP-seq signals around the TSS regions in cells overexpressing SMYD3. D Heat map of SMYD3 ChIP-seq binding profiles around TSS regions (−3 kb to 3 kb) in Huh7-Ctrl and Huh7-SMYD3 cells. E Venn diagram showing the overlap of SMYD3-upregulated genes and genes with SMYD3 binding sites at TSS (−3 kb to 3 kb) regions. F IGV (Integrative Genomics Viewer) profile of SMYD3-enriched regions over the S1PR1 locus which was mainly at the promoter regions (−1 kb to the TSS). G mRNA expression levels of the 11 candidate genes in HepG2/LO2 (immortalized hepatocytes), SMMC-7721/Chang live (immortalized hepatocytes), Sk-Hep1-SMYD3/Sk-Hep1-Ctrl (transiently transfected with SMYD3), and Huh7-SMYD3/Huh7-Ctrl cells (transiently transfected with SMYD3). H qPCR and (I) western blot analyses of S1PR1 expression in HCC cells with stable SMYD3 overexpression (Huh7-Ctrl vs. Huh7-SMYD3 and SK-Hep1-Ctrl vs. SK-Hep1-SMYD3) or stable knockdown (SMMMC-7721-sh-NC vs. SMMMC-7721-sh-SMYD3 and Hep3B-sh-NC vs. Hep3B-sh-SMYD3). Data are presented as the means ± SD of three separate experiments, each performed in triplicate. *P < 0.05; **P < 0.01.
