Fig. 5: SMYD3 regulated its downstream signaling pathways via S1PR1.
From: SMYD3 promotes hepatocellular carcinoma progression by methylating S1PR1 promoters
A IHC staining was performed on paired tumor and adjacent noncancerous tissues from 148 patients with HCC. B Statistical analysis of the IHC scores between HCC and adjacent normal tissues. C Kaplan-Meier analysis of overall survival in patients with HCC stratified according to the S1PR1 expression status. The median expression level of S1PR1 was used as the cut-off. D Clinical samples from the same set of tissues were analyzed in the two groups as indicated. The correlation between SMYD3 and S1PR1 expression was assessed by performing a linear regression analysis. E Western blot analysis of SMYD3, S1PR1, p-AKT, AKT, p-STAT3, STAT3, p-Erk1/2, and Erk1/2 expression levels in control cells and cells overexpressing S1PR1 and cells overexpressing SMYD3 combined with knockdown of SMYD3 or S1PR1. Images are representative of two experiments. Bar = 200 μm. *P < 0.05; **P < 0.01.
