Fig. 4: WFDC21P regulates cell proliferation, migration, and apoptosis. | Cell Death & Disease

Fig. 4: WFDC21P regulates cell proliferation, migration, and apoptosis.

From: miR-4293 upregulates lncRNA WFDC21P by suppressing mRNA-decapping enzyme 2 to promote lung carcinoma proliferation

Fig. 4

a MTT assays of A549 at 24 h post-transfection of pcDNA-WFDC21P and pc-DNA. Data are expressed as mean ± SD for triplicate experiments. **p < 0.01; Student’s t-test. b qRT-PCR analysis of WFDC21P level in si-WFDC21P- and scramble-treated cells. Data are expressed as mean ± SD for triplicate experiments. **p < 0.01; Student’s t-test. c MTT assays of A549 at 24 h post-transfection of si-WFDC21P and scramble. Data are expressed as mean ± SD for triplicate experiments. **p < 0.01; Student’s t-test. d FACS analysis of A549 cell apoptosis at 24 h post-transfection of pc-DNA, pcDNA-WFDC21P, si-WFDC21P, and scrambled control. e Western blot detection of STAT3 and apoptosis-related factors (Bcl-2, Bax, BIRC3, and Caspase 3,8,9) pcDNA vs pcDNA-WFDC21P; scramble vs si-WFDC. f–i Transwell assays of A549 migration. f, g pcDNA vs pcDNA-WFDC21P; (h) and (i) scramble vs. si-WFDC. Data are expressed as mean ± SD for triplicate experiments. **p < 0.01; Student’s t-test. j–m Colony formation assays of A549. j, k pcDNA vs pcDNA-WFDC21P; (l) and (m) scramble vs. si-WFDC. Data are expressed as mean ± SD for triplicate experiments. **p < 0.01; Student’s t-test.

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