Fig. 2: Silencing OGT reduces the toxicity of primary neurons induced by hypercalcemia.

A Western blot analysis was used to detect the expression of OGT in brain tissues of mice in each group (n = 8). * p < 0.05. vs. the control group (mice on a standard diet). B Expression of OGT in neurons induced by hypercalcemia. *p < 0.05. vs. the control group (neurons cultured in basic medium). C Western blot analysis was used to detect the interference efficiency of three OGT interference sequences. *p < 0.05. vs. the si-NC group (neurons treated with si-NC). D luo-4-am, and Perkin Elmer Operetta were used to measure the intracellular calcium level. E Comet assay was used to observe the DNA damage of each group. F Flow cytometry was used to detect apoptosis. G MTS assay was used to detect the neuronal viability. H Western blot analysis was used to detect the expression of apoptosis-related proteins. *p < 0.05. vs. the control group (neurons cultured in basic medium). ^#p < 0.05. vs. the Ca²⁺ + si-NC group (neurons treated with Ca²⁺ + si-NC). The experiment was repeated 3 times independently.