Fig. 3: Silencing OGT attenuates hypercalcemia-induced neurotoxicity by inhibiting EZH2 expression. | Cell Death & Disease

Fig. 3: Silencing OGT attenuates hypercalcemia-induced neurotoxicity by inhibiting EZH2 expression.

From: Silencing of O-linked N-acetylglucosamine transferase ameliorates hypercalcemia-induced neurotoxicity in renal failure by regulating EZH2/KLF2/CXCL1 axis

Fig. 3

A The expression of EZH2 in CKD mice of the GSE148084 dataset. B The expression of EZH2 in brain tissues of CKD mice was detected by Western blot analysis (n = 8). * p < 0.05. vs. the control group (mice on a standard diet). C Western blot analysis was used to detect the expression of EZH2 in neurons induced by hypercalcemia. * p < 0.05. vs. the control group (neurons cultured in basic medium). D RT-qPCR, and Western blot analysis were used to detect the effect of OGT silencing on EZH2 expression. * p < 0.05. vs. neuron treated with si-NC. E Western blot analysis was used to detect the expression changes of OGT and EZH2 after simultaneous silencing of OGT and overexpression of EZH2. F Comet assay was used to observe the DNA damage of neurons in each group. G Flow cytometry was used to detect neuronal apoptosis. H MTS method was used to detect the vitality of neurons in each group. I The expression of apoptosis-related proteins was detected by Western blot. (E–I), *p < 0.05. vs. the si-NC + oe-NC group (neuron treated with si-NC + oe-NC). #p < 0.05. vs. the si-OGT + oe-NC group (neuron treated with si-OGT + oe-NC). The experiment was repeated 3 times independently.

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