Fig. 5: Silencing OGT attenuates hypercalcemia-induced neurotoxicity by regulating the EZH2/KLF2/CXCL1 axis.

A Western blot analysis was used to detect the expression of OGT and CXCL1. B Comet assay was used to observe DNA damage. C Flow cytometry was used to detect apoptosis. D MTS assay was used to detect neuronal viability. E Western blot analysis was used to detect the expression of apoptosis-related proteins. *p < 0.05. vs. the si-NC + oe-NC group (neurons treated with si-NC + oe-NC). #p < 0.05. vs. the si-OGT + oe-NC group (neurons treated with si-OGT + oe-NC). The experiment was repeated 3 times independently.