Fig. 6: Silencing OGT in vivo attenuates hypercalcemia-induced nerve injury in CKD mice.

A Western blot analysis was used to detect the expression of OGT in the brain tissue of each group. B Escape latency and step-down tests were used to observe the changes of nerve function in CKD mice. C Immunofluorescence was used to detect the number of neurons in brain tissues of each group. D TUNEL staining was used to detect neuronal apoptosis. E Western blot analysis was used to detect the expression of EZH2, KLF2, CXCL1, and apoptosis-related proteins. *p < 0.05. vs. the sh-NC group (mice harboring neurons treated with sh-NC). n = 8 mice. The experiment was repeated 3 times independently.